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Ivermectin resistance in dung beetles exposed for multiple generationsuse asterix (*) to get italics
Daniel Gonzalez Tokman, Antonio Arellano Torres, Fernanda Baena-Diaz, Carlos Bustos, Imelda Martinez MPlease use the format "First name initials family name" as in "Marie S. Curie, Niels H. D. Bohr, Albert Einstein, John R. R. Tolkien, Donna T. Strickland"
<p>Ivermectin is an antiparasitic drug commonly used in cattle, that is excreted in dung, causing lethal and sub-lethal effects on coprophagous non-target fauna. Given that cattle parasites generate resistance to ivermectin, farmers have increased the used doses, with a consequent threat to wild fauna. The dung beetle species Euoniticellus intermedius provides ecosystem services by burying dung in cattle pastures, however it is highly threatened by ivermectin. Here we experimentally tested whether E. intermedius generates resistance against ivermectin after being exposed for several generations to a sublethal dose. We generated two laboratory lines where beetles were exposed to either ivermectin-treated or ivermectin-free dung for 18 generations. We compared reproductive success (total brood balls, emerged beetles, proportion emerged and days to emergence) of beetles from both lines across generations. Additionally, for each line, we carried-out toxicity experiments with increasing ivermectin concentrations to determine if sensitivity to ivermectin was reduced after some generations of exposure (i. e. if beetles acquired ivermectin resistance by means of transgenerational effects). Our results show that dung beetles do not generate resistance to ivermectin after 18 generations of continuous exposure and quantitative genetic analyses show low genetic variation in response to ivermectin across generations. Together, these results indicate low potential for adaptation to the contaminant in the short term. Although we cannot exclude that adaptation could occur in the long term, our results and comparative evidence in other insects indicate that dung beetles, and probably other species, are at risk of extinction in ivermectin-contaminated pastures unless they are pre-adapted to tolerate high ivermectin concentrations.</p> should fill this box only if you chose 'All or part of the results presented in this preprint are based on data'. URL must start with http:// or https:// should fill this box only if you chose 'Scripts were used to obtain or analyze the results'. URL must start with http:// or https://, should fill this box only if you chose 'Codes have been used in this study'. URL must start with http:// or https://
antiparasitic, experimental evolution, pesticide resistance, Scarabaeinae
NonePlease indicate the methods that may require specialised expertise during the peer review process (use a comma to separate various required expertises).
Ecosystem Health, Environmental pollution, Global changes, Terrestrial ecotoxicology
Jörg Römbke, Kevin Floate, Jean-Pierre Lumaret, Bernarda Calla, Luis Carlos Martinez, Pierre Jay-Robert suggested: José R. Verdú, Mylène Weill [] suggested: pierrick Labbé (, Mylène Weill [] suggested: Haoues Alout (, Mylène Weill [] suggested: Jean Philippe David ( No need for them to be recommenders of PCI Ecotox Env Chem. Please do not suggest reviewers for whom there might be a conflict of interest. Reviewers are not allowed to review preprints written by close colleagues (with whom they have published in the last four years, with whom they have received joint funding in the last four years, or with whom they are currently writing a manuscript, or submitting a grant proposal), or by family members, friends, or anyone for whom bias might affect the nature of the review - see the code of conduct
noe.g. John Doe []
2023-05-12 04:57:32
Christian Mougin